K-TSTA,淀粉总量检测试剂盒,TotalStarch(AA/AMG)AssayKit

产品识别号:K-TSTA

产品品名:淀粉总量检测试剂盒

英文品名:TotalStarch(AA/AMG)AssayKit

规格型号:100assaysperkit

AOACMethod996.11AACCMethod76-13.01RACIStandardMethodforthemeasurementoftotalstarchincerealfloursandfoodproducts.AACCandAOACapprovedmethod.Thiskitnowcontainsanimprovedα-amylasethatallowstheamylaseincubationstobeperformedatpH5.0(aswellaspH7.0).

INTRODUCTION:

Starchdeterminationmethodsarebroadlygroupedintoacid

hydrolysisorenzymicprocedures.1Acidhydrolysisprocedures

canonlybeappliedtopurestarchsamplesandthushavelimited

application.Enzymicproceduresvaryinpre-treatmentsteps,2starch

gelatinisation,liquefactionanddextrinisation,hydrolysisofdextrinsto

GlucoseandGlucosemeasurement3.AACCMethod76-114specifies

starchgelatinisationunderaqueousconditionsinanautoclave,

followedbystarchconversiontoGlucosewithamyloglucosidaseand

Glucosemeasurement.AACCMethod76-11underestimatesstarch

contentinarangeofsamplesandmaterials,includinghighamylose

maizestarchesandmanyprocessedcerealproducts.Mostmethodsin

usetodayincorporatetreatmentwithThermostableα-amylaseeither

duringorimmediatelyfollowingthestarchgelatinisationstep.5,6For

sampleswhicharedifficulttogelatinise(suchashighamylosemaize

starch)solventssuchassodiumhydroxideordimethylsulphoxide

(DMSO)7,8havebeenemployed.Inaproceduretoensurecomplete

solubilisationofstarchindietaryfibredetermination,Englystand

Cummings(1988)7includedtreatmentwiththestarchdebranching

enzyme,pullulanase.

Tosatisfytheneedforanextremelysimple,yetquantitativeand

reliable,procedureforthemeasurementoftotalstarchMegazyme

produces,andoffers,atotalstarchassaykitbasedontheuseof

thermostableα-amylaseandamyloglucosidase(McClearyetal.9).This

methodhasbeenadoptedbyAOAC(OfficialMethod996.11)and

AACC(Method76.13).

Morerecently,thermostableα-amylasesthatareactiveandstable

atlowerpHvalueshavebecomeavailable.Consequently,wehave

updatedourtotalstarchmethodologytoincorporatesuchan

enzyme10.Themajoradvantageofthisimprovementistoallowboth

thethermostableα-amylaseandamyloglucosidaseincubationsteps

tobeperformedatthesamepH(pH5.0)which,inturn,simplifies

theassayandminimisesthepossibilityofproductionofmaltulose

(4-α-glucopyranosyl-D-fructose)11,whichisresistanttohydrolysisby

amyloglucosidaseandα-amylase.

TheMegazymetotalstarchanalysisprocedureallowsthe

measurementoftotalstarchinawiderangeoffood,feed,plantand

cerealproducts(naturalorprocessed).Formostsamples(e.g.wheat

flour),starchiscompletelysolubilizedonincubatingthesampleat

approx.100°Cinthepresenceofthermostableα-amylase.Samples

containinghighlevelsofresistantstarch(e.g.highamylosemaize

starch),requirepre-dissolutionincold2MKOHorhotDMSO.For

samplescontainingsolublestarchormaltodextrins,cookingwith

thermostableα-amylaseisnotrequired.

KITS:

Kitssuitableforperforming100determinationsoftotalstarchare

availablefromMegazyme.Thekitscontainthefullassaymethodplus:

Bottle1:Thermostableα-amylase(10mL,3,000U/mL

onCeralphareagent*atpH6.5and40°Cor1600

U/mLonCeralphareagentatpH5.0and40°C).

Stablefor大于4yearsat4°C.

Bottle2:Amyloglucosidase(10mL,3300U/mLonsoluble

starch(or200U/mLonp-nitrophenylβ-maltoside*)

atpH4.5and40°C.

Stablefor大于4yearsat4°C.

*Fullassayprocedureisavailableat“www.megazyme.com”.

Bottle3:GOPODReagentBuffer.Buffer(48mL,

pH7.4),p-hydroxybenzoicacidandsodiumazide

(0.4%w/v).

Stablefor大于4yearsat4°C.

Bottle4:GOPODReagentEnzymes.Glucose

oxidaseplusperoxidaseand4-aminoantipyrine.

Freeze-driedpowder.

Stablefor大于5yearsat-20°C.

Bottle5:D-Glucosestandardsolution(5mL,1.0mg/mL)in

0.2%(w/v)benzoicacid.

Stablefor大于4yearsatroomtemperature.

Bottle6:Standardisedregularmaizestarchcontrol.Starch

contentshownonviallabel.

Stablefor大于4yearsatroomtemperature.

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